Making and Using Antibodies: A Practical Handbook, Second Edition

Přední strana obálky
Gary C. Howard, Matthew R. Kaser
CRC Press, 29. 7. 2013 - Počet stran: 458
Antibodies protect us from a wide range of infectious diseases and cancers and have become an indispensable tool in science-both for conventional immune response research as well as other areas related to protein identification analysis. This second edition of Making and Using Antibodies: A Practical Handbook provides clear guidance on all aspects
 

Obsah

Chapter 1 Antibodies
1
Chapter 2 Antigens and Adjuvants
7
Chapter 3 Production of Polyclonal Antibodies
33
Chapter 4 Purification and Characterization of Antibodies
67
Chapter 5 Production of Monoclonal Antibodies
97
Chapter 6 Quantitative Production of Monoclonal Antibodies
119
Chapter 7 Making Antibodies in Bacteria
151
Chapter 8 Selection of Aptamers
173
Chapter 11 Immunohistochemical Methods
303
Chapter 12 Immunoelectron Microscopy
343
Chapter 13 Flow Cytometry
371
Chapter 14 ELISAs
385
Chapter 15 Humanization of Antibodies
395
Chapter 16 Antibodies in the Future
421
Color Insert
429
Back Cover
431

Chapter 9 Chemical and Proteolytic Modification of Antibodies
207
Chapter 10 Western Blots and Other Applications
275

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O autorovi (2013)

Matthew R. Kaser, DPhil, earned his DPhil in biochemistry from Oxford University (UK) in 1988. After postdoctoral positions at the University of California, the University of Texas, and at REI Harbor-UCLA Medical Center, he was appointed to a faculty position at the University of California, San Francisco, Department of Pediatrics, then served as a scientist and patent agent at Incyte Genomics in Palo Alto, California, and a lecturer at California Sate University East Bay, Hayward. Dr. Kaser has been practicing as a patent agent since 1999, was associate director of intellectual property at Mendel Biotechnology, Hayward, and is a senior partner at Bell & Associates in San Francisco. He has presented research papers at a number of regional, national, and international conferences and coauthored more than a dozen publications.

Gary C. Howard, PhD, earned his PhD in biological sciences from Carnegie Mellon University in 1979. He completed his postdoctoral training at Harvard University and The Johns Hopkins University and was a research assistant biochemist at the University of California, San Francisco. He then joined Vector Laboratories in Burlingame as a biochemist and Medix Biotech (a subsidiary of Genzyme) in Foster City, California, as chemistry manager and operations manager. Currently, he is manager of scientific editing at The Gladstone Institutes, a private biomedical research institute affiliated with the University of California, San Francisco.

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