Making and Using Antibodies: A Practical Handbook, Second EditionGary C. Howard, Matthew R. Kaser CRC Press, 29. 7. 2013 - Počet stran: 458 Antibodies protect us from a wide range of infectious diseases and cancers and have become an indispensable tool in science-both for conventional immune response research as well as other areas related to protein identification analysis. This second edition of Making and Using Antibodies: A Practical Handbook provides clear guidance on all aspects |
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Chapter 3 Production of Polyclonal Antibodies | 33 |
Chapter 4 Purification and Characterization of Antibodies | 67 |
Chapter 5 Production of Monoclonal Antibodies | 97 |
Chapter 6 Quantitative Production of Monoclonal Antibodies | 119 |
Chapter 7 Making Antibodies in Bacteria | 151 |
Chapter 8 Selection of Aptamers | 173 |
Chapter 11 Immunohistochemical Methods | 303 |
Chapter 12 Immunoelectron Microscopy | 343 |
Chapter 13 Flow Cytometry | 371 |
Chapter 14 ELISAs | 385 |
Chapter 15 Humanization of Antibodies | 395 |
Chapter 16 Antibodies in the Future | 421 |
Color Insert | 429 |
Back Cover | 431 |
Chapter 9 Chemical and Proteolytic Modification of Antibodies | 207 |
Chapter 10 Western Blots and Other Applications | 275 |
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Making and Using Antibodies: A Practical Handbook Gary C. Howard,Matthew R. Kaser Náhled není k dispozici. - 2006 |
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adjuvant affinity animals antibody production antibody response antigen aptamers ascites assay B-cells binding buffer Bio-IgG Biol biotin biotinylated blocking cell line Centrifuge Chem cloning coli colloidal gold column concentration conjugated containing cross-linking culture detection dilution disulfide bonds dsDNA electrophoresis ELISA elution enzyme epitopes exemplar protocol Figure flow cytometry fluorescent fragments Freund’s gene human hybridoma hybridoma cells IgG1 immune response immunogen immunoglobulin Immunol Methods Incubate injection labeling ligand medium membrane mg/mL mice microscopy modification molecular molecules monoclonal antibodies mouse myeloma nitrocellulose nonspecific optimal papain pellet peptide phage pipette plate polyclonal polyclonal antibodies primary antibody procedure protein purification rabbit reaction reagent recombinant resuspend Rinse room temperature sample secondary antibody sections selection SELEX sequence serum sodium solution specific staining streptavidin supernatant target thiols tion tissue titers transfer tube vector vitro vivo volume wash western blot