Computer Operation for Microscope Photometry
CRC Press, 25. 11. 1997 - Počet stran: 256
Suitable for both microscopists seeking computer skills and PC enthusiasts interested in light microscopy, this interdisciplinary text explores the capabilities of the computer-assisted light microscope. Written in clear, simple language, the book explains how computer technology expands the usefulness of the light microscope in spectrophotometry, fluorometry, polarimetry, spatial scanning, and related fields.
Beginning with the basic features of light microscopy and personal computer interfacing, the text explains how to make photometric measurements and covers spectrophotometry, stepper motors, and server motors. Polarized light and video image analysis complete this introduction to the field. While software examples are provided to illustrate specific techniques, most operations are described as generalized algorithms that can be adapted to any appropriate high-level language, and used with almost any configuration of the microscope. The book suggests new experiments to inspire further study. Promising new areas of interest, such as the use of fluorescence and polarization, are also included.
Computers have radically changed the field of light microscopy in recent decades. Computer Operations for Microscope Photometry helps you master the new techniques.
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Shutters and Apertures
Monochromators and Spectrophotometry
Mechanical Stages and Scanning
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absorbance acceptance window algorithm analyzer angle arc lamp bandpass birefringence buffer camera Chapter collagen condenser cover slip dark-field device dynamic range dynamic range method emission spectrum enter excitation fast shutter fiberoptic field aperture fluid fluorometry GMpc1 GMpc2 GMulti GOSUB GOTO GPIB halogen illuminator shutter increment interface lens light guide light intensity light path magnification maximum dynamic range measuring aperture microscope monochromator mounted muscle fiber numerical aperture objective ocular operator optical axis optical fiber optical window OUTPUT GMpc peak phase photodiode photometer photometric aperture photoresistor pixel PMT output polarized light PRINT prism probe problem refractive index relative rotation sample chamber scanning stage shown in Figure signal specimen spectra spectrophotometry standard stepper motor structure swing-in temperature thermal stage tilting stage tissue transmittance valve voltage wavelength Wollaston prism Zeiss