Making and Using Antibodies: A Practical HandbookGary C. Howard, Matthew R. Kaser CRC Press, 13. 12. 2006 - Počet stran: 408 Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols. Written and edited by an elite team of scienti |
Obsah
Chapter 1 Antibodies | 1 |
Chapter 2 Antigens | 7 |
Chapter 3 Adjuvants | 27 |
Chapter 4 Production of Polyclonal Antibodies | 41 |
Chapter 5 Production of Monoclonal Antibodies | 73 |
Chapter 6 Quantitative Production of Monoclonal Antibodies | 95 |
Chapter 7 Purification and Characterization of Antibodies | 125 |
Chapter 8 Making Antibodies in Bacteria | 157 |
Chapter 10 Applications | 247 |
Chapter 11 Immunohistochemical Methods | 273 |
Chapter 12 Immunoelectron | 315 |
Chapter 13 Flow Cytometry | 339 |
Chapter 14 ELISAs | 361 |
Challenges and Opportunitites | 371 |
| 377 | |
Back cover | 397 |
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Making and Using Antibodies: A Practical Handbook Gary C. Howard,Matthew R. Kaser Náhled není k dispozici. - 2006 |
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acid adjuvant affinity animal antibody production antibody response antigen ascites assay avidin binding buffer Bio-IgG bioreactor biotin biotinylated blocking Centrifuge clones coli colloidal gold column concentration conjugated containing cross-linking culture detection dilution disulfide bonds ELISA elution emulsion enzyme epitopes exemplar protocol Figure flow cytometry fluorescence fragments Freund’s fusion hybridoma hybridoma cells IgG1 immobilized immune response immunogen immunoglobulin Immunol immunolabeling Incubate injection isotype labeling ligand medium membrane method mg/ml mice microscopic modification molecular molecules monoclonal antibodies mouse murine myeloma nitrocellulose nonspecific optimal papain particles pellet peptide peroxidase phage pipette plate polyclonal antibodies primary antibody procedures protein purification rabbit reaction reactive reagent recombinant Rinse room temperature sample secondary antibody sections serum sodium solution specific staining sterile streptavidin supernatant syringe techniques thiols tissue titer transfer tube vector vitro vivo volume wash Western blot µg/ml