In Situ Hybridisation: Application to Developmental Biology and MedicineN. Harris, D. G. Wilkinson Cambridge University Press, 31. 8. 1990 - Počet stran: 288 Advances in our understanding of biological mechanisms have frequently been associated with the development of new techniques. In situ hybridization is a classic case of just such an advance. The technique effectively combines histochemistry with molecular biology and enables the rapid analysis of the distribution of RNA, or DNA, in the tissues. The information gained from this has caused something of a revolution in our understanding of developmental biology, since a fundamental aspect of development is the spatial and temporal expression of genes. In addition the technique has found application in the field of medicine, where it is giving new insights into the functioning of healthy tissues and the diagnosis and study of diseases. This book brings together contributions from leaders in the application of this technique and will help the reader review and be guided through the various options and variations of the technique. |
Obsah
Use of haptenised nucleic acid probes in fluorescent in situ | 33 |
Contributions of the spatial analysis of gene expression to the study | 69 |
Advantages and limitations of in situ hybridisation as exemplified | 97 |
The use of in situ hybridisation to study the localisation of maternal | 115 |
In situ hybridisation in the analysis of genes with potential roles | 131 |
McFadden | 144 |
Localisation of expression of male flowerspecific genes from maize | 157 |
Greenland A | 172 |
Tissue preparation techniques for in situ hybridisation studies | 175 |
Seeds Jealotts Hill Research Station Bracknell Berkshire RG12 6EY | 186 |
Investigation of gene expression during plant gametogenesis by | 189 |
Sexing the human conceptus by in situ hybridisation | 205 |
Nonisotopic in situ hybridisation in human pathology | 241 |
The demonstration of viral DNA in human tissues by in situ | 271 |
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In Situ Hybridisation: Application to Developmental Biology and Medicine N. Harris,D. G. Wilkinson Náhled není k dispozici. - 2011 |
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